Purification and Characterization of Extracellular, Polyextremophilic α-amylase Obtained from Halophilic Engyodontium album
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چکیده
منابع مشابه
Purification and Characterization of Extracellular, Polyextremophilic α-amylase Obtained from Halophilic Engyodontium album
Background: a-Amylases (EC 3.2.1.1) are covering approximately 25% of total enzyme market and are frequently used in food, pharmaceutical and detergent industries. Objectives: The first ever detailed characterization of amylase from any halophilic Engyodontium album is presented. Materials and Methods: An extracellular α-amylase was studied from halophilic E. album TISTR 3645. The enzyme was e...
متن کاملpurification and characterization of extracellular, polyextremophilic α-amylase obtained from halophilic engyodontium album
background: a-amylases (ec 3.2.1.1) are covering approximately 25% of total enzyme market and are frequently used in food, pharmaceutical and detergent industries.objectives: the first ever detailed characterization of amylase from any halophilic engyodontium album is presented. materials and methods: an extracellular α-amylase was studied from halophilic e. album tistr 3645. the enzyme was ext...
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An obligate halophilic Aspergillus gracilis which was isolated from a hypersaline man-made saltern from Thailand was screened for its potential of producing extracellular α -amylase in the previous studies. In this study the α -amylase was extracted and purified by the help of column chromatography using Sephadex G-100 column. Presence of amylase was verified by SDS-PAGE analysis, showing a sin...
متن کاملpurification and characterization of an extracellular thermostable alkaline α-amylase from the moderately halophilic bacterium, bacillus persicus
background: today a large number of bacterial amylases are available commercially in industry. the goal of the present study was purification and biochemical characterization of an extracellular thermostable alkaline α-amylase from the novel moderately halophilic, bacillus persicus from the aran-bidgol, iran. methods : purification of enzyme, was carried out by acetone precipitation, ultrafil...
متن کاملPurification and Characterization of a Polyextremophilic α-Amylase from an Obligate Halophilic Aspergillus penicillioides Isolate and Its Potential for Souse with Detergents
An extracellular α-amylase from the obligate halophilic Aspergillus penicillioides TISTR3639 strain was produced and enriched to apparent homogeneity by ammonium sulfate precipitation and Sephadex G100 gel filtration column chromatography. The mass of the purified amylase was estimated to be 42 kDa by SDS-PAGE. With soluble starch as the substrate it had a specific activity of 118.42 U · mg(-1)...
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ژورنال
عنوان ژورنال: Iranian Journal of Biotechnology
سال: 2014
ISSN: 1728-3043,2322-2921
DOI: 10.15171/ijb.1155